Journal: Bioactive Materials

Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

doi: 10.1016/j.bioactmat.2026.02.018

Figure Lengend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

Article Snippet: Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay.

Techniques: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot

Journal: STAR Protocols

Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

doi: 10.1016/j.xpro.2026.104527

Figure Lengend Snippet: Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.

Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

Techniques: Plasmid Preparation, Sequencing, Virus, Single Cell

Journal: STAR Protocols

Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

doi: 10.1016/j.xpro.2026.104527

Figure Lengend Snippet: Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.

Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

Techniques: Flow Cytometry, Single Cell, RNA Sequencing, Fluorescence, Control